chemical epigenetic drug library  (Cayman Chemical)

Journal: NPJ Precision Oncology

Article Title: Lestaurtinib’s antineoplastic activity converges on JAK/STAT signaling to inhibit treatment naïve and therapy resistant forms ovarian cancer

doi: 10.1038/s41698-025-00947-0

Figure Lengend Snippet: a Viability of indicated cell lines following 48 h of treatment with 1 µM of the 145 compounds contained in the Cayman Chemical epigenetic drug library (#11076) relative to DMSO vehicle. N = 2. b Drug screen hit prioritization schema. Figure created with BioRender.com. c Dose response curves (two-fold dilution starting at 2.5 µM) and IC50 concentrations for lestaurtinib in a panel of therapy-sensitive and -resistant ovarian cancer cell lines following 5–10 days of treatment. N = 8. Graphs represent mean ± standard error. Abbreviations: Cis Res cisplatin-resistant, Olap R olaparib-resistant.

Article Snippet: Fig. 1 Identification of lestaurtinib as a novel inhibitor of therapy-sensitive and -resistant ovarian cancer cells. a Viability of indicated cell lines following 48 h of treatment with 1 μM of the 145 compounds contained in the Cayman Chemical epigenetic drug library (#11076) relative to DMSO vehicle.

Techniques: Drug discovery

Journal: Antibiotics

Article Title: High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression

doi: 10.3390/antibiotics11070933

Figure Lengend Snippet: High throughput screening for HDP inducers. Two stable luciferase reporter cell lines, HTC/ AvBD9-luc ( a ) and HTC/ AvBD10-luc ( b ), were employed to screen a library of 148 epigenetic compounds at the final concentration of 20 µM each. Relative luciferase activity was measured following a 24-h exposure and normalized to the cytotoxicity of each compound, followed by calculation of the strictly standardized mean difference (SSMD) value. Those with a minimum SSMD value of 3 were identified as the hits.

Article Snippet: HTC/ AvBD10-luc cell clone D5 were seeded at 2 × 10 4 cells/well in 50 μL of complete RPMI 1640 medium in 96-well plates overnight prior to exposure to 20 μM of each of the 148 compounds in an Epigenetics Screening Library (Cayman Chemical) in individual wells for 24 h prior to luciferase assay.

Techniques: High Throughput Screening Assay, Luciferase, Concentration Assay, Activity Assay

Journal: Antibiotics

Article Title: High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression

doi: 10.3390/antibiotics11070933

Figure Lengend Snippet: Concentration-dependent activation of the AvBD9 and AvBD10 gene promoters by 33 epigenetic compounds. HTC/ AvBD9-luc ( a ) and HTC/ AvBD10-luc ( b ) luciferase reporter cell lines were stimulated in duplicate with three different concentrations (5, 20, and 80 μM) of each for 24 h, followed by luciferase and cytotoxicity assays. Butyrate (16 mM) and an equal volume of DMSO were used as positive and negative controls, respectively. Fold changes were calculated as relative luciferase activity of a compound divided by that of DMSO-treated cells, after normalization to cell viability. The inset in Panel ( a ) shows the fold changes in the AvBD9 promoter-driven luciferase activity of non-HDAC inhibitors. The results are means ± SEM of three independent experiments.

Article Snippet: HTC/ AvBD10-luc cell clone D5 were seeded at 2 × 10 4 cells/well in 50 μL of complete RPMI 1640 medium in 96-well plates overnight prior to exposure to 20 μM of each of the 148 compounds in an Epigenetics Screening Library (Cayman Chemical) in individual wells for 24 h prior to luciferase assay.

Techniques: Concentration Assay, Activation Assay, Luciferase, Activity Assay

Journal: iScience

Article Title: A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells

doi: 10.1016/j.isci.2022.104153

Figure Lengend Snippet: Generation of human SAN-like cells from hESCs (A) Schematic representation of the differentiation protocols 1–8. The protocol involves three stages (S1, S2, and S3) during the first 9 days of directed differentiation. (B–D) Quantitative PCR (B), live fluorescence images (C), and FACS plots (D) of the day 30 cells derived from the SHOX2:GFP;MYH6:mCherry H9 line using the corresponding differentiation protocols (n = at least 3 biological replicates for each condition). For quantitative PCR data, fold changes were normalized to protocol #1. p values calculated using ordinary one-way ANOVA with Fisher’s test (∗∗p < 0.01, ∗∗∗p < 0.001 ). Data are represented as mean ± SEM. The x axis in panel D is side scatter. (E) Quantitative PCR analysis for SHOX2 transcriptional expression of GFP + cells purified after sorting. n = 3 biological replicates. p value calculated by unpaired two-tailed Student’s t-test ∗∗∗p < 0.001. Data are represented as mean ± SEM, and normalized to GFP-negative population. (F) Schematic and results of the epigenetic library chemical screen. (G) Schematic of the final differentiation protocol. (H) Immunofluorescence image and FACS plot of day 30 cells derived using the protocol described in (G), Scale bar = 100 μm. AA: Activin A, B: BMP4, Ch: CHIR99021, Wi: Wnt inhibitor, RA: Retinoic Acid, Fi: FGF inhibitor, Cu: cucurbitacin, Tyr490: tyrphostin AG 490. See also Figures S1 and .

Article Snippet: Cayman Epigenetic Chemical library , Cayman , #11076.

Techniques: Real-time Polymerase Chain Reaction, Fluorescence, Derivative Assay, Expressing, Purification, Two Tailed Test, Immunofluorescence

Journal: iScience

Article Title: A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells

doi: 10.1016/j.isci.2022.104153

Figure Lengend Snippet:

Article Snippet: Cayman Epigenetic Chemical library , Cayman , #11076.

Techniques: Recombinant, Software